Chromatography trace for protein purification PRODUCTS
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PRELIMINARY SELECTION GUIDE

Step 1. Selection by flow rate
Less than 3mL/minRD2 or RD4 with ultrasonic "debubbler"
3 to 300mL/minRD2 or RD4
10 to 1000mL/minRD2I or RD4I with ultrasonic "debubbler"
100 to 1000mL/minRD2I or RD4I
1 to 10L/minRD2I or RD4I with a splitter
Step 2. Selection by conductivity and pH
Conductivity and pH are not neededRD2 or RD2I
Conductivity is needed but pH is notRD4 or RD4I
Both conductivity and pH are neededRD5 or RD5I
Step 3. Selection by wavelength
This detectors could measure at one or two wavelengths.

Choose the needed value(s) in a particular product page. Standard values are 250 and 280nm (the least expensive). However, other values in range 235nm to 1000nm are possible.

Step 4. Selection by Refractive index
RI could be inexpensively added to any detector.

Please note: because the flow cell is not thermostated (only the internal optics is) the RI provided is not as sensitive as in dedicated HPLC RI detectors. Usually, one can observe 100mg/L (and up) of polyethylene glycol in water. Also, this RI is useful for sucrose gradient separations.
Organic chemists: if you are using TMS or TBDMS protective groups you could monitor the elution of the excess of your silylating reagents, usually hydrolyzed to R3Si-O-SiR3, as a clear RI peak on these detectors.

Step 5. Selection by fraction collection
You are collecting a few large volume fractionsPlease consider ordering valves, as many as your fraction count plus one (usually waste stream). This is EXTREMELY useful if you do the same separation over and over again.

Low hold up volume valves are made entirely from PTFE and are installed underneath of the detectors. Detector can be programmed by an user to open a valve when a set of conditions is met (example: change the flow from waste stream to the second receptacle when optical density at 280nm exceeded say 0.5 OD and 30min had already passed). More about valves

You are collecting many low volume fractions A third party fraction collector can be interfaced to any detector. Please order a specific fraction collector cord.

Any detector can accept "fraction changed" electrical pulse (or an intermittent contact closure) from the fraction collector. The moment of the fraction change is shown on the detector screen as a numbered tick-mark and is also indicated in the data table exported by the detector.
Note: if the manufacturer of a fraction collector does not publish the description of their electrical interface, we might be unable to provide the fraction collector cord. Please check with us if you are planning to buy a third party fraction collector. ISCO Foxy R1/R2 is known to be suitable.

STAND-ALONE CHROMATOGRAPHY MONITORS FOR LABORATORY APPLICATIONS, up to 300 ml/min

Part Number

Press on each cell for full details

Simultaneously monitored parameters*

Starts at

$US
280nm 250nm RI Cond. pH
RD2: 280 Up to 2500 psi (170 bar) 6,565.00
RD2: 250-280 Up to 2500 psi (170 bar) 6,955.00
RD2: 250-280-RI Up to 2500 psi (170 bar) 7,241.00
RD4: 250-280-C Up to 1000 psi (68 bar) 8,132.00
RD4: 250-280-RI-C Up to 1000 psi (68 bar) 8,418.00
RD5: 250-280-C-pH Up to 1000 psi (68 bar) 9,568.00
RD5: 250-280-RI-C-pH Up to 1000 psi (68 bar) 9,854.00
V300 Solenoid Valves Add up to 16 Solenoid Valves

New alternative to fraction collectors

Automatically collect fractions of any volume!

Collection based on signal thresholds and/or time.

324.00

per 1 valve

RD-CA COLUMN ADAPTER Universal Column Adapter Fits all glass columns 104.00

*280nm = UV absorption at 280nm; RI = Refractive Index; Cond. = Conductivity

STAND-ALONE CHROMATOGRAPHY DETECTORS FOR PROCESS DEVELOPMENT

Direct flow rates up to 1 L/min, 1/4” (6.35mm) OD process tubing

Splitter flow rates up to 10 L/min, 1/2” (12.7mm) OD process tubing

Part Number

Press on each row for full details

Simultaneously monitored parameters*

Starts at

$US
280nm 250nm RI Cond. pH
RD2I: 280 Up to 2500 psi (170 bar) 7,287.00
RD2I: 250-280 Up to 2500 psi (170 bar) 7,677.00
RD2I: 250-280-RI Up to 2500 psi (170 bar) 7,963.00
RD4I: 250-280-C Up to 1000 psi (68 bar) 8,853.00
RD4I: 250-280-RI-C Up to 1000 psi (68 bar) 9,139.00
RD5I: 250-280-C-pH Up to 1000 psi (68 bar) 10,290.00
RD5I: 250-280-RI-C-pH Up to 1000 psi (68 bar) 10,576.00
V1500 Solenoid Valves Add up to 16 Solenoid Valves

New alternative to fraction collectors

Automatically collect fractions of any volume!

Collection based on signal thresholds and/or time.

567.00

per 1 valve

RD-SP05 SPLITTER Low Dead Volume Splitter 10 L/min low volume splitter 1,082.00

*280nm = UV absorption at 280nm; RI = Refractive Index; Cond. = Conductivity

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Inquiries: support@reachdevices.com

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