Chromatography trace for protein purification COLUMN ADAPTER


The adapter is used to attach 1/8″ or 1/16″ tubing to a conventional glass columns.

The adapter works by squeezing a flexible O-ring over a glass column outlet.

Because there is no standard size for outlet diameters, three different sizes of O-rings are provided with the adapter. Only one most suitable O-ring should be used at any given time. Extra rings may be placed onto the adapter outer surface for storage (see photo).

Two kinds of O-rings are provided:

  • Viton (brown or black color). Viton O-rings are fairly stable to most solvents. However, prolonged exposure (days) to toluene, DMF, DMSO, and especially a pyridine or amines, will cause excessive, likely permanent swelling or disintegration.
  • Fluorosilicone (blue color) - are adequate for applications involving extensive use of the solvents mentioned above However, fluorosilicone O-rings will swell extensively (but not dissolve or disintegrate) in acetone and to a lesser extent in ethylacetate.

To prolong useful life span of the O-rings, the adapter should not be stored attached to a column soaked with solvents. It is a good practice to rinse the adapter with an appropriate solvent and store it in a dry place.

For most glass columns, 1/8″ tubing is recommended to minimize the hydrodynamic resistance to liquid flow. 1/16″ tubing should be used only for very small glass columns (less than 10 ml volume) to minimize the dead volume of the system.

REACH Devices, LLC.

6525 Gunpark Drive, Suite 370-179, Boulder, CO 80301

Call: (720) 288-5722


Ⓒ 2010-2017 REACH Devices, LLC. All rights reserved.