RD4I SERIES DETECTORS

CHROMATOGRAPHY DETECTOR FOR PROCESS DEVELOPMENT

Simultaneous observation of UV Absorption at 280 and 250nm, Refractive Index and Conductivity

The first chromatography monitor with UV LED light sources and 0.001-800 AU absorption range

UV LED light sources provide over a decade of consistent function while eliminating warm-up time. UV light intensity is temperature-compensated to minimize long-term drift.

Demonstration of Absorption Range 0.001-800 AU at 10mm

Five 1ml samples of mouse mAb in PBS were sequentially eluted through the detector.

The samples contained 0.0001 to 10 mg of the antibody.

EXCELLENT DETECTOR FOR PROTEIN PURIFICATION

Flow rates up tp 1 L/min, accepts 1/4” (6.35 mm) OD process tubing.
Flow rates up to 10 L/min are supported by addition an optional low-volume splitter which accepts plastic or stainless steel 1/2” (12.7 mm) OD process tubing.
Flow Cell is rated to 1000 psi (69 bar).
Total flow cell volume: 400μl
Flow Cell is stable to 1M NaOH, 100% TFA and all organic solvents.
Eluant temperature is recorded
Long-term cold room operation. Compatible with GE Akta, etc.
Can be equipped with a pressure sensor and up to two internal relays (250VAC 4A max)

THE ONLY CHROMATOGRAPHY DETECTOR FOR PROCESS DEVELOPMENT IN ORGANIC SYNTHESIS

Low back-pressure: suitable for gravity columns see graph
Ideal for flash-chromatography
Monitor 1 ng/ml - 10 mg/ml of methyl benzoate (an example for strong chromophore, ε₂₄₂=14400) in a single flow cell
Solvent splash proof anodized titanium case and glass-shielded display
Can be fine-tuned to the particular process by customized firmware upload from a flash drive

INFORMATION ABOUT DATA TRANSFER

LEARN MORE ABOUT SPLITTER

CONVENIENT ALTERNATIVE TO FRACTION COLLECTORS

Up to 8 solenoid valves can be added to all detectors. This allows to automatically collect fractions of unlimited volume into user-supplied vessels. The collection criteria are based on signal thresholds (UV absorption, conductivity, RI or pH), time windows, or a combination of both.

LEARN MORE ABOUT VALVES

PURCHASING

All instruments are sold by Purchase Order only. Standard shipment includes:

The Detector
User Manual
Power cord

Options and additional accessories:

Monitoring Wavelength One (from 235nm to 1000nm)

Monitoring Wavelength Two (from 235nm to 1000nm)

Add Refractive Index

Add valves

Add internal relays

Add pressure sensor

Add low volume Splitter

Fraction collector connection cord

Certificate of origin: Made in USA

TECHNICAL CHARACTERISTICS

LIQUID CONNECTIONS

SS316 1/4" Swagelok-type compression fitting

OPERATION MODE

Autonomous: all unit functions are controlled from the front panel, no external PC is needed.
Up to six parameters are continuously recorded to the detector non-volatile memory.
Up to four parameters can be selected at any time by user to be plotted as real-time graphs on the color LCD.

LIGHT SOURCES

280nm and/or 250nm UV LEDs, followed by narrow bandpass diffraction filters
Spectral bandwidth: 10nm
Wavelength accuracy: ± 3nm
Current through UV LEDs: ≈ 10% of the maximum allowed for the corresponding UV LED. The operating current reading is available on the detector's screen.
Thermally compensated to minimize long-term drift
Typical lifetime: 15 years of non-stop operation

FLOW CELL

A single REACH Devices wide-range embedded flow cell is implemented, US Patent 8570521.
Total flow cell volume: 400μl
Flow rates: 1 to 1000 ml/min, no split - all eluants travel through the flow cell via the same path.
Maximum operating pressure: 1000 psi (68 bar)
Wetted materials: quartz, sapphire, PEEK, titanium, FFKM (Kalrez)
Solvents compatibility: all except HF, concentrated H₂SO₄, concentrated HNO₃, and molecular halogens
Long term pH stability: 0-13
Hydrodynamic resistance: low, see graph

UV MONITORING

Range: 0.001 – 800 AU (Absorption Units)
Resolution: 0.0001 AU
Baseline noise: < 0.0002 AU
Drift: 0.0003 AU/hr at constant temperature (dry flow cell)

CONDUCTIVITY MONITORING

Two bright platinum electrodes
0.01-150 mS/cm range

REFRACTIVE INDEX MONITORING

Measurement type: absolute
Measuring wavelength: 280nm
Range: 1.2000 to 1.7000
Resolution: 0.0001
Thermally compensated measurements

POWER OUTAGE

The recorded data is not corrupted. The detector will automatically resume data recording after the power is back on.

ACQUISITION RATES (non-stop run times)

5Hz (2 hour 38 min)
2Hz (6 hours 37 min)
1Hz (13 hours 14 min)
0.5Hz (26 hours 28 min)
0.2Hz (66 hours 10 min)
0.1Hz (132 hours 20 min)

DATA TRANSFER

By user's command to USB drive. No data acquisition occurs during data transfer.

COMMUNICATION INTERFACE

4 independent analog outputs in 0-1V range (see manual for transfer functions), corresponding to (a) UV 250 nm, (b) UV 280 nm, (c) refractive index, (d) conductivity channels. The output (a) can be reassigned by a user to provide data from any channel and be used to provide the respective analog signal for contemporary fraction collectors.
One digital input pin for fraction count pulse (e.g. from a fraction collector). Detector can react to intermittent mechanical contact closure and TTL level signal.
Two digital outputs which can be programmed by REACH Devices as requested by the user
All input and output channels are galvanically isolated from mains ground and detector's internal circuitry ground for safety and noise resistance.
Up to two relays which can be set by the user to react on change in UV absorption or conductivity.

CUSTOM FIRMWARE UPLOAD

In the field from a flash drive

DISPLAY

Dimensions: 5.7” diagonally
Resolution: QVGA 320x240
Back-light: white LED
Protection: hermetically covered with 2 mm glass – the assembly is solvent-resistant.

CASE MATERIAL

Anodized titanium, covered with 1 micrometer Teflon – complete resistance to any solvent spills.

DIMENSIONS

7.5” x 5.25” x 5.05” (190 x 133 x 128 mm)

WEIGHT

No valves: 4 lb (1.8 kg)
1-8 valves: 4.2 - 5.5 lb (1.9 - 2.5 kg)

POWER REQUIREMENTS

Embedded internal (USA, Asia, and most other regions) or included external (Canada, Europe) power supply
Frequency: 50-60Hz
Voltage: 90-240V
Current rating: 0.1A for 90-120V, or 0.06A for 220-240V; 10W max power draw

Back-pressure graph for RDI Industrial chromatography detectors

RD Industrial chromatography detectors back-pressure graph

REACH Devices, LLC.

6525 Gunpark Drive, Suite 370-179, Boulder, CO 80301

Call: (720) 288-5722

Inquiries: support@reachdevices.com