Chromatography trace for protein purification RD2 SERIES UV MONITORS

UV MONITORS FOR PREPARATIVE CHROMATOGRAPHY and FPLC

Simultaneous observation of UV Absorption at 280 and 250nm, and Refractive Index

The first chromatography monitor with UV LED light sources and 0.001-800 AU absorption range

UV LED light sources provide over a decade of consistent function while eliminating warm-up time. UV light intensity is temperature-compensated to minimize long-term drift.

Demonstration of Absorption Range 0.001-800 AU at 10mm

Five 1ml samples of mouse mAb in PBS were sequentially eluted through the detector.

The samples contained 0.0001 to 10 mg of the antibody.

EXCELLENT UV MONITOR FOR PROTEIN PURIFICATION

THE ONLY CHROMATOGRAPHY DETECTOR FOR ORGANIC AND TOTAL SYNTHESIS

INFORMATION ABOUT DATA TRANSFER
FRACTION COLLECTOR COMMUNICATION

CONVENIENT ALTERNATIVE TO FRACTION COLLECTORS

Up to 16 solenoid valves can be added to all detectors. This allows to automatically collect fractions of unlimited volume into user-supplied vessels. The collection criteria are based on signal thresholds (UV absorption, RI), time windows, or a combination of both.

LEARN MORE ABOUT VALVES

PURCHASING

All instruments are sold by Purchase Order only. Standard shipment includes:

  • The Detector
  • User Manual
  • Power cord
  • 4 feet of 1/16'' PTFE tubing (1/32” bore)
  • 4 feet of 1/8'' PTFE tubing (1/16” bore)
  • Three 1/16” tubing connectors with ferrules, Teflon
  • Three 1/8” tubing connectors with ferrules, Teflon

Options and additional accessories:

Monitoring Wavelength One (from 235nm to 1000nm)

Monitoring Wavelength Two (from 235nm to 1000nm)

Add Refractive Index

Add valves

Adapter to 1.7-2.5 mm ID flexible hose to 1/4”-28TPI male thread connector, PEEK

One male Luer Lock to 1/4”-28TPI male thread connector, PEEK

One female Luer Lock to 1/4”-28TPI male thread connector, PEEK

Fraction collector connection cord

Universal adapter for glass columns, Teflon

Certificate of origin: Made in USA

TECHNICAL CHARACTERISTICS

LIQUID CONNECTIONS

1/4"-28TPI compression fitting to 1/16" or 1/8" OD plastic tubing

OPERATION MODE

  • Autonomous: all unit functions are controlled from the front panel, no external PC is needed.
  • Depending on configuration up to three parameters (UV250, UV280 and RI) plus eluant temperature are continuously recorded to the detector non-volatile memory and are plotted in real-time graphs on a color LCD.

LIGHT SOURCES

  • 280nm and/or 250nm UV LEDs, followed by narrow bandpass diffraction filters
  • Spectral bandwidth: 10nm
  • Wavelength accuracy: ± 3nm
  • Current through UV LEDs: ≈ 10% of the maximum allowed for the corresponding UV LED. The operating current reading is available on the detector's screen.
  • Thermally compensated to minimize long-term drift
  • Typical lifetime: 15 years of non-stop operation

FLOW CELL

  • A single REACH Devices wide-range embedded flow cell is implemented, US Patent 8570521.
  • Total flow cell volume: 30μl
  • Flow rates: 0.1 to 300ml/min, no split - all eluants travel through the flow cell via the same path.
  • Maximum operating pressure: 2500 psi (170 bar)
  • Wetted materials: quartz, sapphire, titanium, FFKM (Kalrez)
  • Solvents compatibility: all except HF
  • Long term pH stability: 0-13
  • Hydrodynamic resistance: see graph

UV MONITORING

  • Range: 0.001 – 800 AU (Absorption Units)
  • Resolution: 0.0001 AU
  • Baseline noise: < 0.0002 AU
  • Drift: 0.0003 AU/hr at constant temperature (dry flow cell)

REFRACTIVE INDEX MONITORING

  • Measurement type: absolute
  • Measuring wavelength: 280nm
  • Range: 1.2000 to 1.7000
  • Resolution: 0.0001
  • Thermally compensated measurements

POWER OUTAGE

The recorded data is not corrupted. The detector will automatically resume data recording after the power is back on.

ACQUISITION RATES (non-stop run times)

  • 5Hz (1 hour 49 min)
  • 2Hz (5 hours 27 min)
  • 1Hz (9 hours 5 min)
  • 0.5Hz (18 hours 11 min)
  • 0.2Hz (45 hours 28 min)
  • 0.1Hz (90 hours 56 min)

DATA TRANSFER

By user's command to USB drive. No data acquisition occurs during data transfer.

COMMUNICATION INTERFACE

  • One digital input pin for fraction count pulse (e.g. from a fraction collector). Detector can react to intermittent mechanical contact closure and TTL level signal.
  • 2 independent analog outputs in 0-1V range (see manual for transfer functions), corresponding to (a) UV 250 nm, and (b) UV 280 nm. The output (a) can be reassigned by a user to provide data from (b) channel and be used to provide the respective analog signal for contemporary fraction collectors.
  • All input and output channels are galvanically isolated from mains ground and detector's internal circuitry ground for safety and noise resistance.

DISPLAY

  • Dimensions: 5.7” diagonally
  • Resolution: QVGA 320x240
  • Back-light: white LED
  • Protection: hermetically covered with 2 mm glass – the assembly is solvent-resistant.

CUSTOM FIRMWARE UPLOAD

In the field from a flash drive

CASE MATERIAL

Anodized titanium, covered with 1 micrometer Teflon – complete resistance to any solvent spills.

DIMENSIONS

  • No valves: 7.5” x 5.25” x 2.25” (190 x 133 x 57 mm)
  • 1-16 valves: 7.5” x 5.25” x 5.05” (190 x 133 x 128 mm)

WEIGHT

  • No valves: 3 lb (<1.4 kg)
  • 1-16 valves: 3.5 - 5 lb (1.6 - 2.3 kg)

POWER REQUIREMENTS

  • Embedded internal (USA, Asia, and most other regions) or included external (Canada, Europe) power supply
  • Frequency: 50-60Hz
  • Voltage: 90-240V
  • Current rating: 0.1A for 90-120V, or 0.06A for 220-240V; 10W max power draw

Back-pressure graph for RD chromatography monitors

RD chromatography detectors backpressure graph

REACH Devices, LLC.

6525 Gunpark Drive, Suite 370-179, Boulder, CO 80301

Call: (720) 288-5722

Inquiries: support@reachdevices.com

Ⓒ 2010-2017 REACH Devices, LLC. All rights reserved.