Protein Purification by Ion-Exchange Chromatography

List of Commercially Available Weak Cation Exchangers^*

Last updated on January 1, 2013. REACH Devices does not manufacture, does not distribute, and does not promote any ion-exchange resins. We develop, manufacture and sell chromatography detectors and detector/gradient pump systems for FPLC, LPLC and MPLC.

2-2.5

2.5-3

3-5

5-7

7-10

10-11

>11

Weak cation exchanger

Carboxymethyl

-O-CH₂-COO^-

3.5-4.5

Z~0

-1<Z<0

Z=-1

^*See main Ion-Exchange Chromatography page for detailed description.

	Diameter, μm: mean (range)	Material	Exclusion limit, (Pore size)	Ionic capacity, μeq/ml	Binding capacity, mg/ml Lysozyme^E (human IgG)^{ELysozyme: pI ~11, MW = 14.5kDa; Human IgG: pI~7.4-8.6, MW = 150kDa; Bovine COHb: MW = 69kDa.}	Max Pressure, psi	pH stability: long term (short term)	Material Trade name
1	400 (300-500)	Agarose, 4% cross-linked, hardened^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	No data	No data	50^{E1No data about the binding conditions.}	9^{F1By analogy with data for Q-Cellthru Bigbeads Plus in A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149–161.}	2-14	CM Cellthru BigBead Plus^01Sterogene
2	128 (80-240)	Dextran, cross-linked^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	30kDa	480-600^{D1Supplier provides ionic capacity data as 4-5 mmol/g. Number in the table obtained by applying the experimentally established in column packing density of 0.12 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ).}	(70 bovine-COHb)^{E2Flow rate of 75 cm/h in 0.1 M acetate buffer, pH 5.0.}	2.5	2-12	CM Sephadex C-25^{02GE Life Sciences (former Amersham biosciences)}
3	No data	Cellulose, microgranular preswollen^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	No data	135-173^{D2Supplier provides ionic capacity data as 0.90-1.15 mmol/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ).}	177^{E3No flow rate reported, 10 mM acetate buffer, pH 5.0. Supplier provides binding capacity data as 1180 mg/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ).}	No data	3-10	CM 52^{03Whatman (part of GE Healthcare)}
4	60-130 fibers	Cellulose, microgranular^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	No data	240^{D3Supplier provides ionic capacity data as 1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.24 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ).}	162^{E1No data about the binding conditions.}	>10	4.5-10^{G1Working pH range.}	Express-Ion C^{03Whatman (part of GE Healthcare)}
5	100 (50-150)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	5,000kDa (1,000Å)	90	25-45^{E1No data about the binding conditions.}	43	2-13	Toyopearl CM-650C^{04TOSOH Biosep LLC (former TosoHaas)}
6	90 [57^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (45–165)	Agarose, 6% crosslinked^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	4,000kDa (546Å)^{C1– Mean pore diameter ISEC-calculated in Ref.3 – P. DePhillips, A.M. Lenhoff, Journal of Chromatography A, 883, 2000, 39–54.}	90–130	(30 bovine-COHb)^{E2Flow rate of 75 cm/h in 0.1 M acetate buffer, pH 5.0.}	43	4-13 (2-14)	CM Sepharose Fast Flow^{02GE Life Sciences (former Amersham biosciences)}
7	73^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.} (40 –130)	Cellulose, cross-linked, beaded^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	>500kDa	187^{D4Supplier provides ionic capacity data as 1.1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.17 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ).}	>70^{E1No data about the binding conditions.}	29	2-12 (1-14)	Cellufine C-500^{05JNC corporation, Japan}
8	(40 –130)	Cellulose, cross-linked, dextran surface extender^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	>1,000kDa	90-220	220^{E4Dynamic at 10 % Breakthrough,}	43	2-13	Cellufine MAX CM^{05JNC corporation, Japan}
9	75 (50-100)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	600kDa (500Å)	160-280	(100 human-IgG)^{E5Flow rate of 212 cm/h, 1mg/ml of polyclonal human Ig G in 50 mM sodium acetate buffer, pH 4.7.}	43	3-13	Toyopearl GigaCap CM-650^{04TOSOH Biosep LLC (former TosoHaas)}
10	67^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.} (40-90)	Methacrylic polymer, cross-linked, proprietary surface extender^{B3Long, linear polymer chains (“tentacles") carry the functional ligands.}	(800Å)	No data	80-120^{E1No data about the binding conditions.}	116	1-13	Fractogel COO (M)^{06EMD Millipore (Merck Millipore)}
11	65 [62^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (40-90)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	5,000kDa (1,000Å)	80-120	30-50^{E1No data about the binding conditions.}	43	2-13	Toyopearl CM-650M^{04TOSOH Biosep LLC (former TosoHaas)}
12	50 [44^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}]	Ceramic scaffold filled w/ polymerized hydrogel^{B4Supplier reports the complete absence of volume changes due to ionic strength or pH.}	No data	250-400	(60 human-IgGkDa)^{E6No flow rate reported, 5 mg/mL Human IgG in 50 mM sodium acetate, pH 4.7.}	1000	2-12 (1-14)	CM Ceramic HyperD F^{07PALL Life Sciences (former BioSepra)}
13	50 [62^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}]	Methacrylic polymer^{B5Supplier reports <4% shrinkage/swelling on change from pure 0.1 to 1M NaCl.}	(1,000Å)	170-250	(>25 Hemo-globin)^{E1No data about the binding conditions.}	102	1-12	Macro-Prep CM^08Bio-Rad.
14	35 (20-40)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	5,000kDa (1,000Å)	80-120	30-50^{E1No data about the binding conditions.}	43	2-13	Toyopearl CM-650S^{04TOSOH Biosep LLC (former TosoHaas)}
15	34 (24-44)	Agarose, highly cross-linked	No data	60-80	No data	>43	4-10^G1(2-14)	CM Sepharose High Performance^{02GE Life Sciences (former Amersham biosciences)}
16	(20-40)	Methacrylic polymer, cross-linked, proprietary surface extender^{B3Long, linear polymer chains (“tentacles") carry the functional ligands.}	(800Å)	No data	120-180^{E1No data about the binding conditions.}	116	1-13	Fractogel COO (S)^{06EMD Millipore (Merck Millipore)}

^A-Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.

^B1-Supplier does not provide data about volume changes due to ionic strength or pH. ^B2-Supplier reports that volume changes due to ionic strength or pH are negligible. ^B3-Long, linear polymer chains (“tentacles") carry the functional ligands. ^B4-Supplier reports the complete absence of volume changes due to ionic strength or pH. ^B5-Supplier reports <4% shrinkage/swelling on change from pure 0.1 to 1M NaCl.

^C1– Mean pore diameter ISEC-calculated in Ref.3 – P. DePhillips, A.M. Lenhoff, Journal of Chromatography A, 883, 2000, 39–54.

^D1-Supplier provides ionic capacity data as 4-5 mmol/g. Number in the table obtained by applying the experimentally established in column packing density of 0.12 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). ^D2-Supplier provides ionic capacity data as 0.90-1.15 mmol/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). ^D3-Supplier provides ionic capacity data as 1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.24 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). ^D4-Supplier provides ionic capacity data as 1.1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.17 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.).

^E-Lysozyme: pI ~11, MW = 14.5kDa; Human IgG: pI~7.4-8.6, MW = 150kDa; Bovine COHb: MW = 69kDa. ^E1-No data about the binding conditions. ^E2-Flow rate of 75 cm/h in 0.1 M acetate buffer, pH 5.0. ^E3-No flow rate reported, 10 mM acetate buffer, pH 5.0. Supplier provides binding capacity data as 1180 mg/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). ^E4-Dynamic at 10 % Breakthrough, ^E5-Flow rate of 212 cm/h, 1mg/ml of polyclonal human Ig G in 50 mM sodium acetate buffer, pH 4.7. ^E6-No flow rate reported, 5 mg/mL Human IgG in 50 mM sodium acetate, pH 4.7.

^F1-By analogy with data for Q-Cellthru Bigbeads Plus in A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149–161.

^G1-Working pH range.

⁰¹-Sterogene ⁰²-GE Life Sciences (former Amersham biosciences) ⁰³-Whatman (part of GE Healthcare) ⁰⁴-TOSOH Biosep LLC (former TosoHaas) ⁰⁵-JNC corporation, Japan ⁰⁶-EMD Millipore (Merck Millipore) ⁰⁷-PALL Life Sciences (former BioSepra) ⁰⁸-Bio-Rad.

Protein Purification by Ion-Exchange Chromatography

List of Commercially Available Weak Cation Exchangers*

List of Commercially Available Weak Cation Exchangers^*