Protein Purification by Ion-Exchange Chromatography

List of Commercially Available Weak Anion Exchangers*

Last updated on January 1, 2013. REACH Devices does not manufacture, does not distribute, and does not promote any ion-exchange resins. We develop, manufacture and sell chromatography detectors and detector/gradient pump systems for FPLC, LPLC and MPLC.

Ion Exchanger type Functional group on IE matrix Charge Z of the functional groups
Abbreviation Name Structure pKa
pH of the solution
<2 2-2.5 2.5-3 3-5 5-7 7-10 10-11 >11
Weak anion exchanger DEAE Diethyl aminoethyl -O-CH2-CH2-NH+(CH2-CH3)2 6-9* Z=+1 0<Z<1 Z=0
DMAE Dimethyl aminoethyl -O-CH2-CH2-NH+(CH3)2 ~10 Z=+1 0<Z<1 Z=0

*See main Ion-Exchange Chromatography page for detailed description.

  Diameter, μm:
mean (range)
Material Exclusion limit, (Pore size) Ionic
capacity, μeq/ml
Binding capacity, mg/ml
LysozymeE
(human IgG)ELysozyme: pI ~11, MW = 14.5kDa; Human IgG: pI~7.4-8.6, MW = 150kDa; Bovine COHb: MW = 69kDa.
Max
Pressure,
psi
pH stability:
long term
(short term)
Material
Trade name
1 400 (300-500) Agarose, 4% cross-linked, hardenedB2Supplier reports that volume changes due to ionic strength or pH are negligible. No data No data 50E1No data about the binding conditions. 9F1By analogy with data for Q-Cellthru Bigbeads Plus in A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149–161. 2-14 DEAE Cellthru BigBead Plus01Sterogene
2 200 (100-300) Agarose with a quartz core, 6% cross-linkedB1Supplier does not provide data about volume changes due to ionic strength or pH. 4,000kDa 130-210 >40E1No data about the binding conditions. 4 2-12 (2-14) Streamline DEAE02GE Life Sciences (former Amersham biosciences)
3 124 (70-220) Dextran, cross-linkedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 30kDa 510-680D1Supplier provides ionic capacity data as 3-4 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.17 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ) (30)E2Flow rate of 75 cm/h in 0.05 M Tris, pH 8.3. 2.5 2-13 DEAE Sephadex A-2502GE Life Sciences (former Amersham biosciences)
4 No data Cellulose, microgranular preswollenB1Supplier does not provide data about volume changes due to ionic strength or pH. No data 160-195D2Supplier provides ionic capacity data in meq/dry gram: 0.88-1.08 for DE-52, 1.8-2.2 for DE-53 and 1.0 for Express-Ion D. Numbers in the table obtained by applying the experimentally established column packing density: 0.18 for both DE-52 and DE-53 and 0.23 for Express-Ion D ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). 130E3No flow rate reported, 10 mM phosphate buffer, pH 8.5. No dataF2Flow of 221 cm/h at 1. 2-9.5 DE 5203Whatman (part of GE Healthcare)
5 No data Cellulose, microgranular preswollenB1Supplier does not provide data about volume changes due to ionic strength or pH. No data 325-400D2Supplier provides ionic capacity data in meq/dry gram: 0.88-1.08 for DE-52, 1.8-2.2 for DE-53 and 1.0 for Express-Ion D. Numbers in the table obtained by applying the experimentally established column packing density: 0.18 for both DE-52 and DE-53 and 0.23 for Express-Ion D ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). 150E3No flow rate reported, 10 mM phosphate buffer, pH 8.5. No dataF3Flow of 137 cm/h at 1. 2-12 DE 5303Whatman (part of GE Healthcare)
6 60-130 fibers Cellulose, microgranularB2Supplier reports that volume changes due to ionic strength or pH are negligible. No data 230D2Supplier provides ionic capacity data in meq/dry gram: 0.88-1.08 for DE-52, 1.8-2.2 for DE-53 and 1.0 for Express-Ion D. Numbers in the table obtained by applying the experimentally established column packing density: 0.18 for both DE-52 and DE-53 and 0.23 for Express-Ion D ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). 60E1No data about the binding conditions. >10 2-9G1Working pH range. Express-Ion D03Whatman (part of GE Healthcare)
7 100 (40–160) Cellulose, beaded B2Supplier reports that volume changes due to ionic strength or pH are negligible. 1,000kDa 110–140 (160)E4Drained medium, 0.5 M Tris, pH 8.3. No dataF4Max linear flow rate 50 cm/h. 2-12 DEAE Sephacel02GE Life Sciences (former Amersham biosciences)
8 100 (90-120) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 50-110 25E5Dynamic at 10 % Breakthrough, 1 mg/mL BSA in 50 mM Tris, pH 8.5. 43F5Flow of 150 -250 cm/h is achieved at 15/ 2-13 Toyopearl DEAE-650C04TOSOH Biosep LLC (former TosoHaas)
9 90 (45–165) Agarose, 6% crosslinkedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 4,000kDa 130–170 (170)E6Flow rate of 300 cm/h, 0.05 M Tris, pH 8.3. 2 3-12 (2-14) DEAE Sepharose CL-6B02GE Life Sciences (former Amersham biosciences)
10 90 [67A– Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.] (45–165) Agarose, 6% crosslinkedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 4,000kDa 110–160 47E7Flow rate of 300 cm/h, 10% breakthrough. 43 2-12 (2-14) DEAE Sepharose Fast Flow02GE Life Sciences (former Amersham biosciences)
11 90 (45-165 ) Agarose, 4% crosslinkedB2, B3Supplier reports that volume changes due to ionic strength or pH are negligible. Contain Diethylaminopropyl group instead of Diethylamiethyl group, providing slightly different selectivity then DEAE Sepharose FF. Pore size distribution of ANX Sepharose 4 Fast Flow (high sub) has been optimized for the separation of large proteins. 30,000kDa 130-170 43E7 43 3-13 (2-14) ANX Sepharose FF (high sub)02GE Life Sciences (former Amersham biosciences)
12 90 (45-165 ) Agarose, 4% crosslinkedB1, B3Supplier does not provide data about volume changes due to ionic strength or pH. Contain Diethylaminopropyl group instead of Diethylamiethyl group, providing slightly different selectivity then DEAE Sepharose FF. Pore size distribution of ANX Sepharose 4 Fast Flow (high sub) has been optimized for the separation of large proteins. No data 60-80 No data No dataF6 3-13 (2-14) ANX Sepharose FF (low sub)02GE Life Sciences (former Amersham biosciences)
13 90 Agarose, cross-linked, dextran surface extenderB1Supplier does not provide data about volume changes due to ionic strength or pH. No data 290-350 No dataE8Surprisingly given not for BSA, but for ovalbumin: 90 mg/ml (Flow rate of 600 cm/h, 10% breakthrough, 0.05 M Tris, pH 8.0). 43 2-12 (2-14) Capto DEAE02GE Life Sciences (former Amersham biosciences)
14 (40 –130) Cellulose, cross-linked, beadedB4Supplier reports 5% shrinkage/swelling on change from pure water to 0.5M NaCl. 30kDa 144D3Supplier provides ionic capacity data in meq/dry gram: 0.9 for Cellufine A-200; 1.3 for Cellufine A-500 and 0.8 for Cellufine A-800. Numbers in the table obtained by applying the experimentally established column packing densities: 0.16 dry g/ml for Cellufine A-200; 0.12 dry g/ml for Cellufine A-500 and 0.10 dry g/ml for Cellufine A-800 ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). >80E1No data about the binding conditions. 29 2-12 (1-14) Cellufine A-20005JNC corporation, Japan
15 (40 –130) Cellulose, cross-linked, beadedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 500kDa 156D3Supplier provides ionic capacity data in meq/dry gram: 0.9 for Cellufine A-200; 1.3 for Cellufine A-500 and 0.8 for Cellufine A-800. Numbers in the table obtained by applying the experimentally established column packing densities: 0.16 dry g/ml for Cellufine A-200; 0.12 dry g/ml for Cellufine A-500 and 0.10 dry g/ml for Cellufine A-800 ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). >60E1No data about the binding conditions. 29 2-12 (1-14) Cellufine A-50005JNC corporation, Japan
16 (40 –130) Cellulose, cross-linked, beadedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 1,000kDa 80D3Supplier provides ionic capacity data in meq/dry gram: 0.9 for Cellufine A-200; 1.3 for Cellufine A-500 and 0.8 for Cellufine A-800. Numbers in the table obtained by applying the experimentally established column packing densities: 0.16 dry g/ml for Cellufine A-200; 0.12 dry g/ml for Cellufine A-500 and 0.10 dry g/ml for Cellufine A-800 ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). >45E1No data about the binding conditions. 29 2-12 (1-14) Cellufine A-80005JNC corporation, Japan
17 (40 –130) Cellulose, cross-linked, dextran surface extenderB1Supplier does not provide data about volume changes due to ionic strength or pH. >1,000kDa 140-250 197E9Dynamic at 10 % Breakthrough, 43 2-12 Cellufine MAX DEAE05JNC corporation, Japan
18 60A– Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. (40-90) Methacrylic polymer, cross-linked, proprietary surface extenderB5Long, linear polymer chains (“tentacles") carry the functional ligands. (800Å) No data 100E10At flow rates of up to 600 cm/h. 116 1-13 Fractogel DEAE (M)06EMD Millipore (Merck Millipore)
19 (40-90) Methacrylic polymer, cross-linked, proprietary surface extenderB5Long, linear polymer chains (“tentacles") carry the functional ligands. (800Å) No data 100E10At flow rates of up to 600 cm/h. 116 1-13 Fractogel DMAE (M)06EMD Millipore (Merck Millipore)
20 59A– Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. (40-90) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 80-120 25E5Dynamic at 10 % Breakthrough, 1 mg/mL BSA in 50 mM Tris, pH 8.5. 44F5Flow of 150 -250 cm/h is achieved at 15/ 2-13 Toyopearl DEAE-650M04TOSOH Biosep LLC (former TosoHaas)
21 50 [59A– Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.] (40-90) Ceramic scaffold filled w/ polymerized hydrogelB6Supplier reports the complete absence of volume changes due to ionic strength or pH. No data >200 >85E11No flow rate reported, 5 mg/mL BSA in 50 mM Tris, pH 8.6. 1000 2-12 (1-14) DEAE Ceramic HyperD F07PALL Life Sciences (former BioSepra)
22 50 [54A– Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.] (25-100) Methacrylic polymerB7Supplier reports <5% shrinkage/swelling on change from pure 0.1 to 1M NaCl. (1,000Å) 100-250 30-40E12flow rates 700-100 cm/h, 3 mg/mL BSA in 10 mM Tris, pH 7.6. 102 1-10 Macro-Prep DEAE08Bio-Rad
23 35 Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 80-120 25E5Dynamic at 10 % Breakthrough, 1 mg/mL BSA in 50 mM Tris, pH 8.5. 44F5Flow of 150 -250 cm/h is achieved at 15/ 2-13 Toyopearl DEAE-650S04TOSOH Biosep LLC (former TosoHaas)
24 30 (20-40) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 50-110 20-40E1No data about the binding conditions. 290 2-13 TSKgel DEAE-5PW (30µm)04TOSOH Biosep LLC (former TosoHaas)
25 (20-40) Methacrylic polymer, cross-linked, proprietary surface extenderB5Long, linear polymer chains (“tentacles") carry the functional ligands. (800Å) No data 100E10At flow rates of up to 600 cm/h. 116 1-13 Fractogel DEAE (S)06EMD Millipore (Merck Millipore)

A– Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.

B1-Supplier does not provide data about volume changes due to ionic strength or pH. B2-Supplier reports that volume changes due to ionic strength or pH are negligible. B3-Contain Diethylaminopropyl group instead of Diethylamiethyl group, providing slightly different selectivity then DEAE Sepharose FF. Pore size distribution of ANX Sepharose 4 Fast Flow (high sub) has been optimized for the separation of large proteins. B4-Supplier reports 5% shrinkage/swelling on change from pure water to 0.5M NaCl. B5-Long, linear polymer chains (“tentacles") carry the functional ligands. B6-Supplier reports the complete absence of volume changes due to ionic strength or pH. B7-Supplier reports <5% shrinkage/swelling on change from pure 0.1 to 1M NaCl.

D1-Supplier provides ionic capacity data as 3-4 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.17 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.) D2-Supplier provides ionic capacity data in meq/dry gram: 0.88-1.08 for DE-52, 1.8-2.2 for DE-53 and 1.0 for Express-Ion D. Numbers in the table obtained by applying the experimentally established column packing density: 0.18 for both DE-52 and DE-53 and 0.23 for Express-Ion D (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). D3-Supplier provides ionic capacity data in meq/dry gram: 0.9 for Cellufine A-200; 1.3 for Cellufine A-500 and 0.8 for Cellufine A-800. Numbers in the table obtained by applying the experimentally established column packing densities: 0.16 dry g/ml for Cellufine A-200; 0.12 dry g/ml for Cellufine A-500 and 0.10 dry g/ml for Cellufine A-800 (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.).

E-Lysozyme: pI ~11, MW = 14.5kDa; Human IgG: pI~7.4-8.6, MW = 150kDa; Bovine COHb: MW = 69kDa. E1-No data about the binding conditions. E2-Flow rate of 75 cm/h in 0.05 M Tris, pH 8.3. E3-No flow rate reported, 10 mM phosphate buffer, pH 8.5. E4-Drained medium, 0.5 M Tris, pH 8.3. E5-Dynamic at 10 % Breakthrough, 1 mg/mL BSA in 50 mM Tris, pH 8.5. E6-Flow rate of 300 cm/h, 0.05 M Tris, pH 8.3. E7-Flow rate of 300 cm/h, 10% breakthrough. E8-Surprisingly given not for BSA, but for ovalbumin: 90 mg/ml (Flow rate of 600 cm/h, 10% breakthrough, 0.05 M Tris, pH 8.0). E9-Dynamic at 10 % Breakthrough, E10-At flow rates of up to 600 cm/h. E11-No flow rate reported, 5 mg/mL BSA in 50 mM Tris, pH 8.6. E12-flow rates 700-100 cm/h, 3 mg/mL BSA in 10 mM Tris, pH 7.6.

F1-By analogy with data for Q-Cellthru Bigbeads Plus in A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149–161. F2-Flow of 221 cm/h at 1. F3-Flow of 137 cm/h at 1. F4-Max linear flow rate 50 cm/h. F5-Value provider in the Tosoh website. Matarial data sheers (downloaded from the same website) give max pressure value ob 102. F5-Flow of 150 -250 cm/h is achieved at 15/

G1-Working pH range.

01-Sterogene 02-GE Life Sciences (former Amersham biosciences) 03-Whatman (part of GE Healthcare) 04-TOSOH Biosep LLC (former TosoHaas) 05-JNC corporation, Japan 06-EMD Millipore (Merck Millipore) 07-PALL Life Sciences (former BioSepra) 08-Bio-Rad